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Jožef Stefan
International
Postgraduate School

Jamova 39
SI-1000 Ljubljana
Slovenia

Phone: +386 1 477 31 00
Fax: +386 1 477 31 10
Email: info@mps.si

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Course Description

Structural Biology of the Cell Cycle

Program

Nanosciences and Nanotechnologies

Lecturers:

prof. dr. Luise Johnson

Goals:

Students become acquainted with the molecular bases of the cell cycle.

Content:

The processes of cell growth and replication are called the cell cycle. To understand the molecular bases of these complex processes is of utmost importance, both from the biological aspect and from the aspect of understanding pathological processes such as cancer which is characterised by unregulated cell growth. This course will deal with the cell cycle particularly from the aspect of the regulation of cyclin-dependent protein kinases, enzymes controlling the start and duration of each cell cycle phase, and will comprise the following thematic packages:
· Cyclin-dependent protein kinases 2 (CDK2): the structure of inactive, cyclin-complexed and phosphorylated forms of kinase, and the structure of active phspho-CDK2
· Structural bases of substrate specificities and its cyclin-dependent binding
· Regulation of CDK by p27 and Ink4 protein inhibitors
· Regulation by other proteins: CK1 and kinase-related phosphatase
· CDK2 as the basis for anti-cancer drug design.

Course literature:

Book:
A. Murray in T. Hunt: The Cell Cycle, W. H. Freeman NY (1993)
Key articles to study:
Johnson, L. N., Noble, M. E. M. & Owen, D. J. (1996) Active and inactive protein kinases. Cell 85, 149-158
Brown, N. R., Noble, M. E. M., Endicott, J. A. & Johnson, L. N. (1999) Structural basis for cyclin dependent kinase substrate and recruitment peptide specificity. Nature Cell Biology 1, 438-443
Song, H., Hanlon, N., Brown, N. R., Noble, M. E. M., Johnson, L. N. & Barford, D. (2001) Phospho-protein/protein interactions revealed by the crystal structure of Kinases Associated Phosphatase (KAP) in complex with CDK2. Molecular Cell, 7, 615-626
Johnson, L. N. & Lewis, R. J. (2001) The Structural basis for control by phosphorylation. Chemical reviews. 101, 2209-2242

Significant publications and references:

Johnson, L. N., Noble, M. E. M. & Owen, D. J. (1996) Active and inactive protein kinases. Cell 85, 149-158
Noble, M. E. M., Endicott, J. A., Brown, N. R. and Johnson, L. N. (1997) The cyclin box fold: protein recognition in cell cycle and transcription control. Trends in Biochem. Sci. 22, 482-487
Brown, N. R., Noble, M. E. M., Endicott, J. A. & Johnson, L. N. (1999) Structural basis for cyclin dependent kinase substrate and recruitment peptide specificity. Nature Cell Biology 1, 438-443
Song, H., Hanlon, N., Brown, N. R., Noble, M. E. M., Johnson, L. N. & Barford, D. (2001) Phospho-protein/protein interactions revealed by the crystal structure of Kinases Associated Phosphatase (KAP) in complex with CDK2. Molecular Cell, 7, 615-626
Johnson, L. N. & Lewis, R. J. (2001) The Structural basis for control by phosphorylation. Chemical reviews. 101, 2209-2242

Examination:

Examination and term paper.

Students obligations:

Preparation of a positively rated term paper and a successfully passed examination .

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