Učni načrt predmeta

Predmet:
Praktikum iz biomaterialov
Course:
Biomaterials Practicum
Študijski program in stopnja /
Study programme and level		 Študijska smer /
Study field		 Letnik /
Academic year		 Semester /
Semester
Nanoznanosti in nanotehnologije, 3. stopnja / 1 1
Nanosciences and nanotechnologies, 3rd cycle / 1 1
Vrsta predmeta / Course type
Izbirni / Elective
Univerzitetna koda predmeta / University course code:
NANO3-946
Predavanja
Lectures		 Seminar
Seminar		 Vaje
Tutorial		 Klinične vaje
work		 Druge oblike
študija		 Samost. delo
Individ. work		 ECTS
15 15 15 105 5

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:
doc. ddr. Marija Vukomanović
Sodelavci / Lecturers:
Jeziki / Languages:
Predavanja / Lectures:
slovenščina, angleščina
Vaje / Tutorial:
Slovenian, English
Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:
Prerequisites:

Zaključen študij druge stopnje s področja naravoslovja ali tehnologije ali zaključen študij druge stopnje na drugih področjih z znanjem osnov s področja predmeta.

Completed second-cycle studies in natural sciences or technology, or completed second-cycle studies in other fields with knowledge of the fundamentals of the subject area.

Vsebina:
Content (Syllabus outline):

Predmet Praktikum iz biomaterialov obravnava praktično znanje, potrebno za oceno interakcij med biomateriali in celicami (vključno s celicami sesalcev in mikrobi). Predavanja bodo predstavila osnovne lastnosti biomaterialov v povezavi z njihovimi specifičnimi in nespecifičnimi interakcijami z živo snovjo. Poseben poudarek bo na pridobivanju osnovnih izkušenj, potrebnih za kvantifikacijo interakcij med biomateriali in celicami ter izogibanju lažno pozitivnim rezultatom. Študenti bodo pridobili osnovno znanje o naslednjih vidikih biomaterialov in njihovih interakcijah z živim okoljem, vključno z:
• Pregled: fizikalno-kemijske lastnosti biomaterialov
• Interakcije med biomaterialom in celicami: specifične in nespecifične interakcije, lastnosti biomaterialov, ki so moteče za običajne biološke teste
• Odziv biomaterialov na biološki sistem: biorazgradnja
• Biomateriali, ki se odzivajo na dražljaje iz okolice: mehanski, električni, magnetni, pH in drugi dražljaji
• Živi biomateriali: biomateriali, ki vsebujejo žive celice
• Biomateriali biološkega izvora: biomateriali, ki jih proizvajajo žive celice
• Tehnike sušenja, ki ohranjajo strukturo medsebojno delujočih sistemov biomaterial/celica
• Interakcije biomateriala s celicami: preiskave z elektronsko mikroskopijo
• Interakcije biomaterialov s celicami: preiskave s fluorescenčno mikroskopijo
• Bioreaktorski sistemi: bolj realistično testiranje interakcij med celicami in biomateriali

The course Biomaterials Practicum addresses the practical knowledge essential for evaluating interactions between biomaterials and cells (including mammalian cells and microbes). The lectures will introduce the fundamental properties of biomaterials in relation to their specific and non-specific interactions with living matter. Particular emphasis will be placed on acquiring the basic level of experience needed for quantifying biomaterial–cell interactions and avoiding false-positive results. Students will gain foundational knowledge on the following aspects of biomaterials and their interactions with living systems, including:
• Overview: physicochemical properties of biomaterials
• Biomaterial–cell interactions: specific and non-specific interactions, properties of biomaterials that interfere with common biological assays
• Response of biomaterials to biological systems: biodegradation
• Stimuli-responsive biomaterials: mechanical, electrical, magnetic, pH and other stimuli
• Living biomaterials: biomaterials containing live cells
• Bio-sourced biomaterials: biomaterials produced by living cells
• Drying techniques that preserve the structure of interacting biomaterial–cell systems
• Interactions of biomaterials with cells: electron microscopy investigations
• Interactions of biomaterials with cells: fluorescence microscopy investigations
• Bioreactor systems: more realistic testing of cell–biomaterial interactions

Temeljna literatura in viri / Readings:

Knjige / Books:
Essential Biomaterials Science, David Williams, Cambridge Texts in Biomedical Engineering, 2014. ISBN: 9781139026086. https://doi.org/10.1017/CBO9781139026086
Biomaterials from Nature for Advanced Devices and Therapies, Nuno M. Neves, Rui L. Reis, 2016. ISBN: 9781119126218. DOI: 10.1002/9781119126218
Biointerfaces: Where Material Meets Biology, Dietmar Hutmacher, Wojciech Chrzanowski, 2014. ISBN: 978-1-78262-845-3. https://doi.org/10.1039/9781782628453
Cryopreservation and Freeze-Drying Protocols, Willem F. Wolkers, Harriëtte Oldenhof, 2021. ISBN: 978-1-0716-0782-4. https://doi.org/10.1007/978-1-0716-0783-1

Revije / Journals:
Nature Biotechnology; Biomaterials; Acta Biomaterialia; Advanced Healthcare Materials; Biomaterials Science; Colloids and Surfaces B: Biointerfaces

Cilji in kompetence:
Objectives and competences:

Glavni cilj predmeta je pridobitev novega znanja o fizikalno-kemijskih lastnostih biomaterialov, razumevanje dvostranskih interakcij med biomateriali in celicami / zunajceličnim matriksom ter napovedovanje vpliva teh interakcij na obe interakcijski strani in njihova uporaba pri praktičnem eksperimentalnem delu.

S tem povezani cilji so:
• Razumevanje fizikalno-kemijske narave biomaterialov
• Spoznavanje praktičnih vidikov nespecifičnih interakcij
• Razumevanje modifikacij biomaterialov v bioloških okoljih
• Poseben poudarek na odzivih biomaterialov na različne okoljske dražljaje
• Poudarjanje biomaterialov, ki vsebujejo žive celice
• Raziskovanje biomaterialov, ki jih tvorijo žive celice
• Spoznavanje pomena tehnike sušenja za ohranjanje občutljivih struktur, ki nastanejo med interakcijami biomaterialov in celic
• Poglobljeno poznavanje elektronskomiikroskopskih vidikov interakcij med biomateriali in celicami
• Poglobljeno poznavanje fluorescenčnomikroskopskih vidikov interakcij med biomateriali in celicami
• Uvod v bolj realistične študije interakcij med biomateriali in celicami

Glavni cilj je povezan z naslednjimi kompetencami:
• Kritično mišljenje pri razlagi biomaterialov in njihovih interakcij z živim okoljem
• Sklepi, ki temeljijo na dokazih, in vrednotenje rezultatov ob upoštevanju možnih interakcij
• Pridobivanje izkušenj z interdisciplinarnimi in transdisciplinarnimi pristopi
• Oblikovanje strokovnega znanja na področju znanosti o biomaterialih
• Načrtovanje eksperimentalnega dela z napovedovanjem možnih interakcij
• Razvoj praktičnih veščin za uspešno eksperimentalno delo

The main objective of the course is to develop new knowledge in the physicochemical properties of biomaterials, to understand the two-sided interactions of biomaterials with cells and the extracellular matrix, to predict the influence of these interactions on both interacting sides, and to apply this knowledge during practical experimental work.

Connected objectives are:
• Understanding the physicochemical nature of biomaterials
• Recognizing practical aspects of non-specific interactions
• Understanding the modifications of biomaterials in biological environments
• Special focus on the responses of biomaterials to different environmental stimuli
• Highlighting biomaterials containing living cells
• Exploring biomaterials made by living cells
• Understanding the importance of drying techniques for preserving delicate structures formed during biomaterial–cell interactions
• Deeper knowledge of electron microscopy aspects of biomaterial–cell interactions
• Deeper knowledge of fluorescence microscopy aspects of biomaterial–cell interactions
• Introduction to more realistic studies of biomaterial–cell interactions

The main objective is connected with the following competences:
• Critical thinking in explaining biomaterials and their interactions with living environments
• Evidence-based conclusions and evaluation of results, taking into account possible interactions
• Gaining experience in interdisciplinary and transdisciplinary approaches
• Developing expertise in biomaterials science
• Planning experimental work by predicting possible interactions
• Developing hands-on laboratory skills for successful experimental work

Predvideni študijski rezultati:
Intendeded learning outcomes:

Študenti bodo pridobili teoretično znanje, ilustrirano s praktičnimi primeri, ki bodo prikazani v resničnem okolju v celičnem in mikrobiološkem laboratoriju. S tem bodo pridobili podrobnejšo sliko o biomaterialih in prve praktične izkušnje, ki bodo osnova za nadaljnje individualno raziskovalno delo. Nova znanja bodo vključevala:
• Osnove fizikalno-kemijskih značilnosti biomaterialov
• Razumevanje spremenljivosti narave biomaterialov v življenjskem okolju
• Zavedanje o obstoju nespecifičnih interakcij pri testiranju biomaterialov v živem okolju
• Pričakovanje odziva biomaterialov na okoljske dražljaje
• Oblikovanje biomaterialov, izdelanih iz živih celic
• Oblikovanje biomaterialov, ki vsebujejo žive celice
• Praktične izkušnje s tehnikami sušenja, ki ohranjajo strukturo interakcijskega sistema biomaterial–celica
• Praktične izkušnje s pripravo in preučevanjem interakcij med biomateriali in celicami z uporabo elektronske mikroskopije
• Praktične izkušnje s pripravo in preučevanjem interakcij med biomateriali in celicami z uporabo fluorescenčne mikroskopije

Students will gain theoretical knowledge illustrated with practical examples that will be demonstrated in a real environment in a cell culture and microbiology laboratory. This will provide them with a more detailed picture of biomaterials and their first practical experience, which will serve as the foundation for further individual research work. New knowledge will include:
• Fundamentals of the physicochemical characteristics of biomaterials
• Understanding the variability of the nature of biomaterials in the living environment
• Awareness of the existence of non-specific interactions when testing biomaterials in the living environment
• Anticipation of the response of biomaterials to environmental stimuli
• Designing biomaterials made from living cells
• Designing biomaterials containing living cells
• Practical experience with drying techniques that preserve the structure of the biomaterial–cell interaction system
• Practical experience with the preparation and study of biomaterial–cell interactions using electron microscopy
• Practical experience with the preparation and study of biomaterial–cell interactions using fluorescence microscopy

Metode poučevanja in učenja:
Learning and teaching methods:

• Predavanja, ki bodo uvedla teoretična izhodišča
• Praktično delo v laboratoriju

• Theoretical background introduced through lectures
• Practical work in the laboratory

Načini ocenjevanja:
Delež v % / Weight in %
Assesment:
Ustni izpit: 3 izbrana vprašanja
50 %
Oral exam: 3 selected questions
Individualna izvedba naloge v laboratoriju
50 %
Individual performance of the laboratory task
Reference nosilca / Lecturer's references:
1. VUKOMANOVIĆ, Marija, et al. Filler-enhanced piezoelectricity of poly-L-lactide and its use as a functional ultrasound-activated biomaterial. Small. 2023, 19, 2301981. DOI: 10.1002/smll.202301981
2. VUKOMANOVIĆ, Marija, et al. Multi-doped apatite: strontium, magnesium, gallium and zinc ions synergistically affect osteogenic stimulation in human mesenchymal cells important for bone tissue engineering. Biomaterials Advances. 2022, 140, 213051-1–213051-11. DOI: 10.1016/j.bioadv.2022.213051
3. VUKOMANOVIĆ, Marija, et al. Antimicrobial activity of piezoelectric polymer: piezoelectricity as the reason for damaging bacterial membranes. Biomaterials Science. 2022, 10, 4933–4948. DOI: 10.1039/d2bm00644h
4. VUKOMANOVIĆ, Marija, et al. Development of a ternary cyclodextrin–arginine–ciprofloxacin antimicrobial complex with enhanced stability. Communications Biology. 2022, 5, 1234-1–1234-13. DOI: 10.1038/s42003-022-04197-9
5. VUKOMANOVIĆ, Marija, et al. Nano-engineering stable contact-based antimicrobials: chemistry at the interface between nano-gold and bacteria. Colloids and Surfaces B: Biointerfaces. 2021, 208, 112083-1–112083-11. DOI: 10.1016/j.colsurfb.2021.112083