MPŠ
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Jožef Stefan
International
Postgraduate School

Jamova 39
SI-1000 Ljubljana
Slovenia

Phone: +386 1 477 31 00
Fax: +386 1 477 31 10
Email: info@mps.si

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Course Description

Microbial Biosensors: Cells and Cellular Component, Populations and Communities

Program

Sensor Technologies

Lecturers:

doc. dr. Aleš Lapanje

Goals:

Objectives:
• to understand versatility and reasonable use of microoorganisms and their components in sensor technologies from molecular up to the community level of organisation,
• is able to critically evaluate measurements based on microorganisms (e.g. mutability, adaptation, toxicity etc.),
• to understand concept of bioavailability and is able to determine quality of measured data particularly from environmental measurements,
• to understand peculiarity of integration of microbial biosensors and their components with aparatures,
• utilise methods for obtaining new sensing components from unculturable microroganisms.

Competency:
• integration of microbial biosensors within complex devices and their evaluation based on specificity, dinamic range and sensitivity,
• interpretation and utilisation of obtained data from microbial biosensors,
• utilise methods for obtaining novel sensing components from microorganisms.

Content:

• Types of microbial biosensors.
• Advantages and disadvantages of properties and use of microbial biosensors.
• Principles of functioning of the components of biosensors which are based on cellular parts (DNA, RNA, proteins).
• Principles of functioning of biosensor devices based on microbial cells and their components by determination of sensing components, transducers and effectors.
• Solutions of common problems in devices based on the microbial biosensors – challenges for mechatronic approaches.
• Novel emerging technologies – use of MFC (Microbial fuel cells) and their components.
• Defining microbial communities as the sensing components of biosensors, techniques of use of analysis of microbial communities – rapid genomic and metagenomic screening and implementation of tools of computation biology.
• Development of new techniques for extracting new receptors from nonculturable microbial cells.
• Microorganisms as an analytes: detection of microorganisms and their quantification.

Course literature:

Books:
• Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems. Zourob, Mohammed; Elwary, Sauna; Turner, Anthony P. F. (Eds.), 2008, Springer.
• Metagenomics: Current Innovations and Future Trends. Diana Marco (Eds.), 2011, Caister Academic Press.
• Chemical Sensors and Biosensors: Microbial Biosensors for Environmental Applications. Renι Lalauze, Gιrald Thouand, Marie Josι Duran, 2013, John Wiley & Sons.

Periodicals:
• Biosensors and Bioelectronics, Elsevier.
• Analytical Chemistry, ACS.
• Biotechnology and applied biochemistry, Wiley.
• Microbiome, BMC.

Significant publications and references:

Bibliography:
• articles: 26,
• citations: 166,
• h index 7,
• patents: 4.

• KOVAČ VIRŠEK, Manca, HUBAD, Barbara, LAPANJE Aleš, Aquatic Toxicology, 2013, vol. 144, str. 208 – 217.
• HUBAD, Barbara, LAPANJE, Aleš. Inadequate hospital ventilation system increases the risk of nosocomial Mycobacterium tuberculosis. J. hosp. infect., 2012, vol. 80, no. 1, str. 88-91.
• FAVET, Jocelyne, LAPANJE, Aleš, GIONGO, Adriana, KENNEDY, Suzanne. Microbial hitchhikers on intercontinental dust : catching a lift in Chad. ISME j. (Print), 2013, vol. 7, issue 4, str. 850-867.
• ZRIMEC, Jan, KOPINČ, Rok, RIJAVEC, Tomaž, ZRIMEC, Tatjana, LAPANJE, Aleš. Band smearing of PCR amplified bacterial 16S rRNA genes: dependence on initial PCR target diversity. J. microbiol. methods. [Print ed.], Nov. 2013, vol. 95, no. 2, str. 186-194.

Examination:

Seminar work (20%)
Oral exam (5%)
Project defence – solving a case (75%)

Students obligations:

Seminar work.
Oral exam.
Project defence – solving a case.

Links: