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Jožef Stefan
International
Postgraduate School

Jamova 39
SI-1000 Ljubljana
Slovenia

Phone: +386 1 477 31 00
Fax: +386 1 477 31 10
Email: info@mps.si

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Course Description

Quantitation of Nucleic Acids

Program

Nanosciences and Nanotechnologies, third-level study programme

Lecturers:

prof. dr. Kristina Gruden

Goals:

Autonomous in quantification of nucleic acids related case studies.

Content:

- Theoretical basis of different quantitative methods.
- Real-time PCR: absolute/relative quantification, reference materials, precision, measurement uncertainty.
- High throughput approaches, multiplexing.
- Linear and isothermal amplifications.
- Different applications: diagnostics, on-site detection, gene expression.
- Case studies.

Course literature:

Review articles, chosen each year specifically.

Significant publications and references:

MORISSET, Dany, DEMŠAR, Tina, GRUDEN, Kristina, VOJVODA, Jana, ŠTEBIH, Dejan, ŽEL, Jana. Detection of genetically modified organisms-closing the gaps : to the editor. Nat. biotechnol., 2009, vol. 27, no. 8, 9 str.

Examination:

Project – case study.

Students obligations:

Lectures and practical courses will be given in an interchangeable manner – first a theory on certain topic will be given, followed by practical examples that will be carried out by the students. For the individual work each student will design an experiment and anticipate the analysis workflow for his chosen use case. Students will present their use cases to each other in a form of a short seminar.

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