Učni načrt predmeta

Predmet:

Biologija matičnih celic

Course:

Biology of Stem Cells

Študijski program in stopnja / Study programme and level	Študijska smer / Study field	Letnik / Academic year	Semester / Semester
Nanoznanosti in nanotehnologije, 3. stopnja	Bioznanosti	1	1
Nanosciences and Nanotechnologies, 3rd cycle	Biosciences	1	1

Vrsta predmeta / Course type

Izbirni / Elective

Univerzitetna koda predmeta / University course code:

NANO3-791

Predavanja Lectures	Seminar Seminar	Vaje Tutorial	Klinične vaje work	Druge oblike študija	Samost. delo Individ. work	ECTS
15	15			15	105	5

*Navedena porazdelitev ur velja, če je vpisanih vsaj 15 študentov. Drugače se obseg izvedbe kontaktnih ur sorazmerno zmanjša in prenese v samostojno delo. / This distribution of hours is valid if at least 15 students are enrolled. Otherwise the contact hours are linearly reduced and transfered to individual work.

Nosilec predmeta / Course leader:

doc. dr. Metka Novak

Sodelavci / Lecturers:

doc. dr. Barbara Breznik Vittori

Jeziki / Languages:

Predavanja / Lectures:

slovenščina, angleščina / Slovenian, English

Vaje / Tutorial:

slovenščina, angleščina / Slovenian, English

Pogoji za vključitev v delo oz. za opravljanje študijskih obveznosti:

Prerequisites:

Predmet lahko vpišejo študentje, ki so uspešno opravili izpit iz Biologije celice ali Biokemija raka na dodiplomskem ali podiplomskem študiju.

Students that have successfully passed the exam of Cell Biology or Biochemistry of cancer in undergraduate or postgraduate studies can enrol.

Vsebina:

Content (Syllabus outline):

I. Matične celice v razvoju in regeneraciji
1. Embriogeneza in embrionske matične celice (biokemijski in biološki procesi razvoja)
2. Inducirane matične celice – indukcija pluripotence
3. Matične celice – etika in politika

II. Matične celice v bolezenskih stanjih
1. Potencialna uporabnost matičnih celic v raznih boleznih - splošno
2. Rakave matične celice

Biology of Stem Cells
I. Stem cells in development and regeneration
1. Embryogenesis and embryonic stem cells (biochemical and biological processes)
2. Induced stem cells – pluripotency induction
3. Stem cells - ethics and politics

II. Stem cells in disease
1. Potential of stem cells application in various diseases - general
2. Cancer stem cells

Temeljna literatura in viri / Readings:

-NOVAK, Metka, MAJC, Bernarda, SKOBLAR VIDMAR, Marija, KLADNIK, Špela, KOLENC MILAVEC, Tina, HABIČ, Anamarija, GALUN, Simona Katrin, ŽIŽEK, Pia, PORČNIK, Andrej, MATJAŠIČ, Alenka, ZUPAN, Andrej, JERALA, Miha, BOŠNJAK, Matic, PRESTOR, Borut, BREZNIK VITTORI, Barbara. Trametinib-mediated MEK inhibition impairs cancer cell viability and invasion in patient-derived tumor avatars of glioblastoma with extraneural metastases : a :proof-of-concept study. Biomedicine & pharmacotherapy. Sept. 2026, vol. 202, [article no.] 119832, str. 1-14, ilustr. ISSN 1950-6007. https://doi.org/10.1016/j.biopha.2026.119832, DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije, https://d.cobiss.net/repository/si/files/287525635/181741/Novak-et-al,-Case-study-2026.pdf, DOI: 10.1016/j.biopha.2026.119832.
-HABIČ, Anamarija, KOLENC MILAVEC, Tina, ŽIŽEK, Pia, KLADNIK, Špela, MAJC, Bernarda, SENJOR, Emanuela, PERIŠIĆ, Milica, PORČNIK, Andrej, PRESTOR, Borut, ŠVAJGER, Urban, NOVAK, Metka, BREZNIK VITTORI, Barbara. Glioblastoma–natural killer cell crosstalk: insights from dynamic spheroid models reveal the importance of secreted cytokines and the CD155 axis. Cell communication and signaling. 2026, vol. 24, [article no.] 289, str. 1-22, ilustr. ISSN 1478-811X. https://link.springer.com/article/10.1186/s12964-026-02826-y, DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije, https://d.cobiss.net/repository/si/files/278556675/179038/s12964-026-02826-y.pdf, DOI: 10.1186/s12964-026-02826-y.
-NOVAK, Metka, MAJC, Bernarda, MALAVOLTA, Marta, PORČNIK, Andrej, MLAKAR, Jernej, HREN, Matjaž, HABIČ, Anamarija, MLINAR, Mateja, JOVCHEVSKA, Ivana, ŠAMEC, Neja, ZOTTEL, Alja, SKOBLAR VIDMAR, Marija, VIPOTNIK-VESNAVER, Tina, ZUPAN, Andrej, MATJAŠIČ, Alenka, TRKOV, Saša, GEORGIEV, Dejan, SADIKOV, Aleksander, BOŠNJAK, Roman, PRESTOR, Borut, KOMEL, Radovan, LAH TURNŠEK, Tamara, BREZNIK VITTORI, Barbara. The Slovenian translational platform GlioBank for brain tumour research : identification of molecular signatures of glioblastoma progression. Neuro-oncology advances. 2025, vol. 7, iss. 1, [article no.] vdaf015, str. 1-15, ilustr. ISSN 2632-2498. https://academic.oup.com/noa/article/7/1/vdaf015/7972534?login=false, DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije, Repozitorij Univerze v Ljubljani – RUL, https://d.cobiss.net/repository/si/files/224100099/150391/vdaf015.pdf, DOI: 10.1093/noajnl/vdaf015.
-BLAŽIČ, Anja, MAJC, Bernarda, NOVAK, Metka, BREZNIK VITTORI, Barbara, REMS, Lea. Invasive properties of patient-derived glioblastoma cells after reversible electroporation in vitro. Radiology and oncology. [Print ed.]. Dec. 2025, vol. 59, no. 4, str. 533-550, viii, ilustr. ISSN 1318-2099. https://reference-global.com/article/10.2478/raon-2025-0058, https://www.radioloncol.com/index.php/ro/article/view/4701, Repozitorij Univerze v Ljubljani – RUL, http://www.dlib.si/details/URN:NBN:SI:doc-HY1RPCMQ, DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije, https://d.cobiss.net/repository/si/files/265177347/181920/535_550_Blazic_Rems_4701_RadiologyAndOncology.pdf, DOI: 10.2478/raon-2025-0058.
-BREZNIK VITTORI, Barbara, KO, Meng-Wei, TSE, Christopher, CHEN, Po-Chun, SENJOR, Emanuela, MAJC, Bernarda, HABIČ, Anamarija, ANGELILLIS, Nicolas, NOVAK, Metka, ŽUPUNSKI, Vera, MLAKAR, Jernej, NATHANSON, David, JEWETT, Anahid. Infiltrating natural killer cells bind, lyse and increase chemotherapy efficacy in glioblastoma stem-like tumorospheres. Communications biology. 2022, vol. 5, str. 1-15, ilustr. ISSN 2399-3642. https://doi.org/10.1038/s42003-022-03402-z, https://www.arrs.si/sl/promocija/odlicni/inc/22/odlicni-22-3.pdf, DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije, https://d.cobiss.net/repository/si/files/107677443/140373/s42003-022-03402-z_107677443.pdf, DOI: 10.1038/s42003-022-03402-z.
-NOORDEN, Cornelis J. F. van, BREZNIK VITTORI, Barbara, NOVAK, Metka, DIJCK, Amber J. van, TANAN, Saloua, VITTORI, Miloš, BOGATAJ, Urban, BAKKER, Noëlle, KHOURY, Joseph D., MOLENAAR, Remco J., HIRA, Vashendriya V. V. Cell biology meets cell metabolism and bone marrow : energy production is similar in stem cells and in cancer stem cells in brain. The Journal of histochemistry and cytochemistry. 2022, vol. 70, iss. 1, str. 29-51, ilustr. ISSN 0022-1554. https://journals.sagepub.com/doi/pdf/10.1369/00221554211054585, DOI: 10.1369/00221554211054585.

Cilji in kompetence:

Objectives and competences:

Študentje:
- se seznanijo z osnovnimi lastnostmi različnih vrst matičnih celic
- razumejo sodobne molekularne tehnike v raziskavah matičnih celic
- razumejo vlogo matičnih celic pri raku

Students acquire:
- knowledge of basic characteristics of different types of stem cells
- understanding of modern molecular techniques in stem cell research
- understanding of the role of stem cells in cancer

Predvideni študijski rezultati:

Intendeded learning outcomes:

Študentu je potrebno:
- izkazati razumevanje molekularne biologije in biokemijskih tehnik, ki se uporabljajo pri raziskavah matičnih celic
- izkazati znanje o bioloških metodah matičnih celic in molekularnih mehanizmih, ki vodijo k pluripotenci in celični diferenciaciji
- razumevanje prednosti in slabosti celične terapije
- analiziranje in interpretacija znanstvene literature

The student needs to:
- Demonstrate an understanding of molecular biology and biochemistry techniques used in stem cell research
- Demonstrate knowledge of stem cell biology and molecular mechanisms leading to pluripotency and cell differentiation
- Understand advantages and disadvantages of cell-based therapeutics
- Master analysis and interpretation of primary scientific literature

Metode poučevanja in učenja:

Learning and teaching methods:

- Osnove predmeta, podane v sklopu predavanj
- Posamezna vroča poglavja študentje samostojno obdelajo v obliki seminarja
- Samostojni projekt

- Lectures on subject basics
- Student seminar preparation on certain currently hot topics
- Independent project

Načini ocenjevanja:

Delež v % / Weight in %

Assesment:

Ustni izpit iz splošnega dela

20 %

Oral exam of the basic theoretical part

Ocena od seminarja

30 %

Seminar assessment

Samostojni projekt

50 %

Independent project

Reference nosilca / Lecturer's references:

1.	MAJC, Bernarda, HABIČ, Anamarija, MALAVOLTA, Marta, VITTORI, Miloš, PORČNIK, Andrej, BOŠNJAK, Roman, MLAKAR, Jernej, MATJAŠIČ, Alenka, ZUPAN, Andrej, SKOBLAR VIDMAR, Marija, LAH TURNŠEK, Tamara, SADIKOV, Aleksander, BREZNIK, Barbara, NOVAK, Metka. Patient-derived organoids mimic glioblastoma resistance to therapy and increase DNA Damage Response. iScience. [Online ed.]. 2023, vol. , no. , str. [1-10]. ISSN 2589-0042. DOI: 10.2139/ssrn.4545256
2.	NOVAK, Metka, KOPRIVNIKAR KRAJNC, Miha, HRASTAR, Barbara, BREZNIK, Barbara, MAJC, Bernarda, MLINAR, Mateja, ROTTER, Ana, PORČNIK, Andrej, MLAKAR, Jernej, STARE, Katja, PESTELL, Richard G., LAH TURNŠEK, Tamara. CCR5-mediated signaling is involved in invasion of glioblastoma cells in its microenvironment. International journal of molecular sciences. 12 Jun. 2020, vol. 21, iss. 12, str. 1-20, ilustr. ISSN 1422-0067
3.	ZOTTEL, Alja, NOVAK, Metka, ŠAMEC, Neja, MAJC, Bernarda, COLJA, Sara, KATRAŠNIK, Mojca, VITTORI, Miloš, HRASTAR, Barbara, ROTTER, Ana, PORČNIK, Andrej, LAH TURNŠEK, Tamara, KOMEL, Radovan, BREZNIK, Barbara, JOVCHEVSKA, Ivana (avtor, korespondenčni avtor). Anti-vimentin nanobody decreases glioblastoma cell invasion in vitro and in vivo. Cancers. 2023, vol. 15, no. 3, str. 1-14, ilustr. ISSN 2072-6694
4.	LAH TURNŠEK, Tamara, MAJC, Bernarda, NOVAK, Metka, SUŠNIK, Ajda, BREZNIK, Barbara, PORČNIK, Andrej, BOŠNJAK, Roman, SADIKOV, Aleksander, MALAVOLTA, Marta, HALILČEVIĆ, Selma, MLAKAR, Jernej, ZOMER, Roby. The cytotoxic effects of cannabidiol and cannabigerol on glioblastoma stem cells may mostly involve GPR55 and TRPV1 signalling. Cancers. 2022, vol. 14, iss. 23, str. 1-21, ilustr. ISSN 2072-6694
5.	HABIČ, Anamarija, NOVAK, Metka, MAJC, Bernarda, LAH TURNŠEK, Tamara, BREZNIK, Barbara. Proteases regulate cancer stem cell properties and remodel their microenvironment. Journal of histochemistry and cytochemistry. [Online ed.]. 2021, vol. 69, iss. 12, str. 775-794. ISSN 1551-5044.