COURSES

Goals

The course is intended to prepare students for research work in the field of quantitative analysis of nucleic acids. After successful completion of the course, the student acquires competences: - student comprehends theory behind quantification of nucleic acids, - gets acquainted with various relevant tools, - is autonomous in solving quantification of nucleic acids related case studies.

Curriculum

- Theoretical basis of different quantitative methods. - Real-time PCR: absolute/relative quantification, reference materials, precision, measurement uncertainty. - High throughput approaches, multiplexing. - Linear and isothermal amplifications. - Digital PCR. - Different applications: diagnostics, on-site detection, gene expression. - Case studies.