COURSES

Goals

General Competences: - the student will master research methods and procedures and develop skills for critical assessment of his activities, - the student will be able to put his knowledge into practice, - the student will develop communications skills to present research achievement in the international environment, - training for team work (in international environment) Course Specific Competences: Student will learn theoretical background of proteomics and its application in biomedical research. During his study he will use the newest scientific literature, which will improve his foreign language skills and his ability to critically view the published research.

Curriculum

Protein separation techniques: sample preparation, PAGE, 2D-PAGE, DIGE, HPLC, MudPIT, affinity chromatography. Mass spectrometry: basic principles of mass spectrometry, types and components of mass spectrometers, MALDI, ESI, CID, ETD, ECD. Quantitative proteomics: ICAT, SILAC, iTRAQ, absolute quantification Bioinformatics: statistical data evaluation, protein and genomic databases, identification of MS/MS spectra, “de novo” sequencing Proteomics and biology: “top down” and “bottom up” approach, protein posttranslational modifications, protein complexes, biomarkers.